Directed evolution of enzymes: Library screening strategies

Directed evolution of industrial enzymes.

Directed evolution of glycosylating enzymes

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CADEE: Computer-Aided Directed Evolution of Enzymes

The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of...

Directed evolution of enzymes and biosynthetic pathways.

Directed evolution is an important tool for overcoming the limitations of natural enzymes as biocatalysts. Recent advances have focused on applying directed evolution to a variety of enzymes, such as epoxide hydrolase, glyphosate N-acetyltransferase, xylanase and phosphotriesterase, in order to improve their activity, selectivity, stability and solubility. The focus has also shifted to manipula...

Directed variation in evolution strategies

Biological evolution gives rise to self-organizing phenomena. Inspired by this theory, directed variation is added to the ( , ) evolution strategies (ES) algorithm and it is called directed variation ES (DVES). In DVES, some neighboring individuals in the population mutate correlatively according to the distribution of the whole population. Experimental results showed that, with the same number...